PREMIER TECHNOLOGY DEVELOPMENT AND SERVICE CENTER FOR COCOA BIOTECHNOLOGY

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MOLECULAR BIOLOGY: WORKING WITH DNA

LIBRARIES

1. cDNA Library Titering, Screening, and Clone Isolation
2. Construction of a Yeast Genomic Library
3. Double-Stranded cDNA Synthesis for Cloning
4. Inverse PCR of YAC Ends
5. Isolation of λ EMBL3/4 Phage DNA
6. M13 Library Construction from BAC or Bacteriophage P1 Clones
7. P1 Bacterial Artificial Chromosome (BAC) Isolation
8. Quick Plasmid Screen of Recombinant Transformants in E.coli
9. Radiolabeling RNA Probes with Polynucleotide Kinase for Screening of cDNA Libraries
10. Screening Genomic or cDNA Libraries with Radiolabeled DNA Probes
11. Screening Phage Libraries with Digoxigenin-Labeled Probes
12. Yeast Chromosome Preparation #1
13. Yeast Chromosome Preparation #2









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