PREMIER TECHNOLOGY DEVELOPMENT AND SERVICE CENTER FOR COCOA BIOTECHNOLOGY

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MOLECULAR BIOLOGY: WORKING WITH DNA

POLYMERASE CHAIN REACTION

1. 3' Rapid Amplification of cDNA Ends (RACE) by PCR
2. 5' Rapid Amplification of cDNA Ends (RACE) by PCR
3. Asymmetric PCR and Single-Sided PCR
4. Basic Polymerase Chain Reaction (PCR) Instructions
5. Core Sample PCR
6. Generation of a Probe for cDNA Cloning by PCR Using Peptide Sequence Information
7. High Fidelity DNA Amplification by PCR using Vent, Pwo, and Pfu DNA Polymerases
8. High Fidelity PCR Amplification Using Vent Polymerase
9. Identifying Different Clones of scFv Using Polymerase Chain Reaction
10. Long-Range Polymerase Chain Reaction Reagents and Guidelines
11. Molecular Profiling: Loss of Heterozygosity
12. Mouse Tail Tip Digest Protocol: PCR from Tail Tip DNA
13. Mouse Tail Tip Digest Protocol: Preparation of DNA
14. PCR Amplification of Yeast Open Reading Frames (ORFs) with Yeast GenePairs® Primers from Research Genetics for Microarray Experiments
15. PCR from Whole Yeast
16. PCR Gene Splicing by Amplification of VH and VL Genes to create scFv Gene Repertoires
17. PCR Protocol for PCR-Mediated Gene Disruption
18. Random Mutagenesis by PCR




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