PREMIER TECHNOLOGY DEVELOPMENT AND SERVICE CENTER FOR COCOA BIOTECHNOLOGY

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MOLECULAR BIOLOGY: WORKING WITH DNA

VECTORS

1. A Rapid Boiling Method for the Preparation of Bacterial Plasmids
2. Agrobacterium Plasmid DNA Miniprep
3. Chloramphenicol Treatment of DH-α-5 Cells for Amplification of Low Copy Number Plasmids
4. Introduction to Vector Design for the Generation of Transgenic Mice
5. Liquid Lysate Lambda Phage Miniprep
6. M13 Library Construction from BAC or Bacteriophage P1 Clones
7. Phage DNA Purification with DE52 Cellulose
8. Phage Purification
9. Plasmid Rescue from P[lacZ] Lines in Drosophila
10. Preparation of Single Stranded Plasmid DNA
11. Quick Preparation of Plasmid DNA from Yeast
12. Screening of Cosmid Libraries and Isolation of Positive Colonies
13. Subcloning to Isolate End Fragments of Cosmids for Chromosome Walking









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