<HTML>
<body bgcolor=white>
<blockquote>

<basefont color=#ff0000>

<p align="center">
<A href="http://koko.gov.my"><img border="0" src="LOGO3.gif" width="100" height="100">
<A href="CCBR3.html"><img border="0" src="banerinm5.jpg" width="90" height="100">

<font face="Arial" color=green size="-1">
<h4><Marquee>PREMIER TECHNOLOGY DEVELOPMENT AND SERVICE CENTER FOR COCOA BIOTECHNOLOGY</Marquee>
</h4>

<center>
<A href="index.htm"><font face="Arial" color=#0000FF size="-1">Home</FONT></A> | 
<A href="Profile.html"><font face="Arial" color=#0000FF size="-1">Profile</FONT></A> | 
<A href="CCBR3.html"><font face="Arial" color=#0000FF size="-1">Achievement</FONT></A> | 
<A href="Research.html"><font face="Arial" color=#0000FF size="-1">Programmes</FONT></A> | 
<A href="Program.html"><font face="Arial" color=#0000FF size="-1">Projects</FONT></A> |
<A href="Staff.html"><font face="Arial" color=#0000FF size="-1">Staffs</FONT></A> | 
<A href="Publication.html"><font face="Arial" color=#0000FF size="-1">Publications</FONT></A> | 
<A href="Journals.html"><font face="Arial" color=#0000FF size="-1">Journals</FONT></A> |<br> 
<A href="Glossary.html"><font face="Arial" color=#0000FF size="-1">Biotech Glossary</FONT></A> |
<A href="Bioinformatics.html"><font face="Arial" color=#0000FF size="-1">Bioinformatics</FONT></A> | 
<A href="Protocol1.html"><font face="Arial" color=#0000FF size="-1">Lab Protocol</FONT></A> | 
<A href="Notes.html"><font face="Arial" color=#0000FF size="-1">Notes</FONT></A> | 
<A href="University.html"><font face="Arial" color=#0000FF size="-1">Malaysia University</FONT></A> | 
 
			<p> 
</TD></TR></TABLE><p>
</center>
<font size=2>
<font color="black">

<center>
<A href="Protocol1.html"><font face="Arial" color=BLACK size=3>MOLECULAR BIOLOGY: WORKING WITH DNA</FONT></A><p>
<A href="Cloning.html"><font face="Arial" color=#0000FF size=3>CLONING</FONT></A><p>

</CENTER>
<font size=2>

           

 
1. <a href="Cloning1.html"><font face="Arial" color=#0000FF size="-1">3' Rapid Amplification of cDNA Ends (RACE) by PCR  </FONT></A><br>
2. <a href="Cloning2.html"><font face="Arial" color=#0000FF size="-1">DNA Ligation  </FONT></A><br>
3. <a href="Cloning3.html"><font face="Arial" color=#0000FF size="-1">Double-Stranded cDNA Synthesis for Cloning</FONT></A><br>  
4. <a href="Cloning4.html"><font face="Arial" color=#0000FF size="-1">End-Filling DNA with Taq Polymerase  </FONT></A><br>
5. <a href="Cloning5.html"><font face="Arial" color=#0000FF size="-1">Filling 5' Extensions to Create Flush Termini for Cloning (Klenow Reaction)</FONT></A><br>  
6. <a href="Cloning6.html"><font face="Arial" color=#0000FF size="-1">Generation of a Probe for cDNA Cloning by PCR Using Peptide Sequence Information  </FONT></A><br>
7. <a href="Cloning7.html"><font face="Arial" color=#0000FF size="-1">Labeling Oligonucleotides Using T4 Polynucleotide Kinase  </FONT></A><br>
8. <a href="Cloning8.html"><font face="Arial" color=#0000FF size="-1">Ligation of Phosphorylated Linkers to Blunt-Ended Target Fragments  </FONT></A><br>
9. <a href="Cloning9.html"><font face="Arial" color=#0000FF size="-1">M13 Library Construction from BAC or Bacteriophage P1 Clones  </FONT></A><br>
10. <a href="Cloning10.html"><font face="Arial" color=#0000FF size="-1">Nested Deletions Using Exonuclease III and Mung Bean Nuclease  </FONT></A><br>
11. <a href="Cloning11.html"><font face="Arial" color=#0000FF size="-1">Plasmid Rescue from P[lacZ] Lines in Drosophila  </FONT></A><br>
12. <a href="Cloning12.html"><font face="Arial" color=#0000FF size="-1">Subcloning DNA Fragments into Plasmids - Ligation with T4 DNA Ligase</FONT></A><br>  
13. <a href="Cloning13.html"><font face="Arial" color=#0000FF size="-1">Trimming 3' Extensions to Create Flush Termini for Cloning (T4 DNA Polymerase Reaction) </FONT></A><br> 
<p><br><P><BR><P><BR><P><BR><P><BR><P><BR><P><BR><HR>
<center><font size=1><i>Cited in http://www.bio.com/protocolstools/browsdesc.jhtml