Biotech Glossary |
Bioinformatics |
Lab Protocol |
Notes |
Malaysia University |
2. Nco I and Not I Restriction Digestion of the scFv Gene Repertoires
3. Amino-allyl Reverse Transcription
4. Dephosphorylation of DNA Using Calf Intestine Alkaline Phosphatase
5. DNA Ligation
6. End-Filling DNA with Taq Polymerase
7. Extended Sequencing with Klenow Fragment
8. Extended Sequencing with Klenow Fragment Using Double-Stranded DNA as Template
9. Filling 5' Extensions to Create Flush Termini for Cloning (Klenow Reaction)
10. General Protocol: Restriction Enzyme Digest of DNA
11. Labeling of DNA Probes using Primer Extension
12. Labeling Oligonucleotides
13. Labeling Oligonucleotides Using T4 Polynucleotide Kinase
14. Ligation in Low-Melting Temperature Agarose Gel
15. Ligation of Phosphorylated Linkers to Blunt-Ended Target Fragments
16. Nested Deletions Using Exonuclease III and Mung Bean Nuclease
17. Nick Translation Labeling of DNA with γ-[32P]-dNTP
18. Partial Restriction Digest of DNA
19. Preparation and Purification of Taq Polymerase
20. Preparation of Topoisomerase I from Drosophila Embryos
21. Radiolabeling of Oligonucleotides
22. Reverse Transcriptase Assay Optimized for Avian Sarcoma Virus
23. Reverse Transcriptase Assay Optimized for Molonoy Murine Leukemia Virus (MuLV)
24. Screening Genomic or cDNA Libraries with Radiolabeled DNA Probes
25. Subcloning DNA Fragments into Plasmids - Ligation with T4 DNA Ligase
26. The Incremental Truncation for the Creation of Hybrid EnzYmes (ITCHY) Protocol using α-Phosphothioate Nucleotide Analogs
27. The Incremental Truncation for the Creation of Hybrid EnzYmes (ITCHY) Protocol
28. Trimming 3' Extensions to Create Flush Termini for Cloning (T4 DNA Polymerase Reaction)