Biotech Glossary |
Bioinformatics |
Lab Protocol |
Notes |
Malaysia University |
2. Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants
3. EMS (Ethylmethane Sulfonate) Mutagenesis of Yeast #1
4. EMS Mutagenesis of Yeast #2
5. Ethylmethane Sulfonate (EMS) Mutagenesis of Arabidopsis
6. Generation of Uni-Directional Nested Deletions in Plasmid DNA
7. Hydroxylamine Mutagenesis of Plasmid DNA
8. In Vitro Mutagenesis with E. coli dut ung Single Stranded DNA
9. Kunkel Method of Site-Directed Mutagenesis
10. Oligonucleotide-Directed Mutagenesis
11. Plasmid Rescue from P[lacZ] Lines in Drosophila
12. Preparation of Single-Stranded DNA for Site-Directed Mutagenesis
13. Production of Uracil-Containing Single Stranded DNA (ssDNA) Template
14. Random Mutagenesis by PCR
15. Removal of Selectable Marker Cassettes from Transfected ES clones using Cre-Lox Mediated Recombination
16. Site-Directed Mutagenesis of Double Stranded DNA by Elimination of a Unique Restriction Enzyme Site
17. Site-Directed Mutagenesis Using a Single-Stranded DNA Template
18. Synthesis of Specific Sequence Using Mutually Priming Long Oligonucleotides
19. The Incremental Truncation for the Creation of Hybrid EnzYmes (ITCHY) Protocol using α-Phosphothioate Nucleotide Analogs
20. The Incremental Truncation for the Creation of Hybrid EnzYmes (ITCHY) Protocol
21. X-Ray Mutagenesis of Drosophila