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1. 2D Gel Assay for RNA-Binding Proteins
2. Autoradiography
3. Casting, Preparing, and Running SDS-Polyacrylamide Mini-Gels
4. Gel Mobility Shift Assay Using Magnesium Chloride and EDTA
5. Immobilized pH Gradient (IPG) As First Dimension for 2D Protein Gels-Large Scale Protein Application
6. Immunoprecipitation of Proteins from Radiolabeled Cultures
7. Isolation and Renaturation of Proteins from SDS-PAGE Gels
8. Isolation of Polypeptides from Polyacrylamide Gels for Immunoprecipitation Assays
9. Native Gel Electrophoresis of RNA-Protein Complexes
10. Preparation of Gradient Gels - General Protocol
11. Preparation of Tissue Samples for SDS PAGE and Detection of Proteins by Immunoblot
12. Rapid Yeast Protein Preparation for SDS-PAGE and Western Analysis
13. Running Novex NuPage Gels
14. SDS-PAGE of Immobilized pH Gradient (IPG) Strips for the Second Dimension
15. SDS-PAGE Sample Preparation of Adenovirus Infected Cells
16. Trichloroacetic Acid (TCA) Precipitation of Radiolabeled Protein and Quantification by Scintillation Spectroscopy
17. Triton-Acetic Acid-Urea (TAU) Gel Electrophoresis of Histones
18. Use of Immobilization pH Gradient (IPG) Strips for First Dimension of 2D Protein Gels

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