PREMIER TECHNOLOGY DEVELOPMENT AND SERVICE CENTER FOR COCOA BIOTECHNOLOGY

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MOLECULAR BIOLOGY: WORKING WITH DNA

SEQUENCING

1. Buffer Gradient Denaturing Gels
2. Complementary Oligonucleotides and Nested Deletions for Linker Scanning
3. Double Stranded DNA Sequencing Template Preparation
4. Extended Sequencing with Klenow Fragment
5. Nested Deletions Using Exonuclease III and Mung Bean Nuclease
6. Preparation of Acrylamide Urea Gels
7. Preparation of Sequencing Gels














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